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KMID : 0545120180280071209
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 7 p.1209 ~ p.1216
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Antimicrobial effect of 2-phenylethynyl-butyltellurium in Escherichia coli and its association with oxidative stress
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Pinheiro Franciane Cabral
Bortolotto Vandreza Cardoso
Araujo Stifani Machado
Poetini Marcia Rosula
Sehn Carla Pohl
Neto Jose S. S.
Zeni Gilson
Prigol Marina
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Abstract
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This study aimed to evaluate the antimicrobial activity of 2-phenylethynyl-butyltellurium (PEBT) in Escherichia coli and the relation to its pro-oxidant effect. For this, we carried out disk diffusion test, minimum inhibitory concentration (MIC) assay, and survival curve analysis. We also measured the level of extracellular reactive oxygen species (ROS), activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), and level of non-protein thiols (NPSH). PEBT at 1.28 and 0.128 mg/disk exhibited antimicrobial capability in disk diffusion test, with MIC value of 1.92 mg/ml, whereas PEBT at 0.96, 1.92, and 3.84mg/ml inhibited bacterial growth after a 9-hour exposure. PEBT at 3.84, 1.92, and 0.96mg/ml increased extracellular ROS production, decreased intracellular NPSH level, and reduced SOD and CAT activities. GSH and AA in the medium protected the bacterial cells from the antimicrobial effect of PEBT. In conclusion, PEBT exhibited antimicrobial activity against E. coli, involving the generation of ROS, oxidation of NPSH, and reduction of the antioxidant defenses in the bacterial cells.
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KEYWORD
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Antibacterial, antioxidant enzymes, tellurium, pro-oxidant activity, oxidative stress, thiol groups
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